| 质粒名称: | pORANGE Cloning template vector |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 131471 |
| 存储实验室: | Harold MacGillavry |
|
载体骨架基本信息 |
|
| 载体名称: | pORANGE |
| 空载体大小: | 8494bp |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | HA (N terminal on insert) |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SpCas9 |
| 物种来源: | S. pyogenes |
| 启动子: | CMV |
| 标签/融合蛋白: | U6 and Cbh |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | 未知(未破坏) |
| 3’克隆位点: | 未知 (未破坏) |
| 5’测序引物 | aaggctgttagagagataattggaa |
| 3’测序引物 | cgggccatttaccgtaagtt |
|
文献引用方法 |
|
| 材料和方法部分: | pORANGE Cloning template vector was a gift from Harold MacGillavry (Addgene plasmid # 131471 ; http://n2t.net/addgene:131471 ; RRID:Addgene |
| 参考文献部分: | ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD. PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. 10.1371/journal.pbio.3000665 Pub |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pORANGE Cloning template vector 8494 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Cloning template form ORANGE method based knock-in constructs.
ACCESSION .
VERSION .
KEYWORDS pORANGE Cloning template vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8494)
AUTHORS Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE,
Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD
TITLE ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope
tagging of endogenous proteins in neurons.
JOURNAL PLoS Biol. 2020 Apr 10;18(4):e3000665. doi:
10.1371/journal.pbio.3000665.
PUBMED 32275651
REFERENCE 2 (bases 1 to 8494)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8494
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 62..302
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 62..82
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 233..252
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 329..404
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 559..844
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 846..1123
/label=chicken beta-actin promoter
intron 1124..1351
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
CDS 1372..1398
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1402..1422
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1432..5532
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5536..5556
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(5596..5613)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5602..5809
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 5818..5958
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5818..5947
/label=AAV2 ITR
rep_origin 6033..6488
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6120..6139)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6330..6351
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6505..6524)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6624..6646
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6684..6702)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6770..6874
/gene="bla"
/label=AmpR promoter
CDS 6875..7735
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7093..7112)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7906..8494
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8395..8414
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
61 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
121 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
181 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
241 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
301 acgaaacacc gggtcttcga gaagacctgt tttagagcta gaaatagcaa gttaaaataa
361 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gttttagagc
421 tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca attctgcaga
481 caaatggctc tagaagcttg ctagcaattg tcgactcgag gatcctagga cgcgtcctag
541 gacgcgtcca gaggtacccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
601 caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc attgacgtca
661 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
721 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt gtgcccagta
781 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
841 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
901 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
961 gggggggcgc gcgccggggg gggggggggg gggggggggg gggggsgggg sgrggcggag
1021 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
1081 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
1141 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
1201 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagct
1261 gagcaagagg taagggttta agggatggtt ggttggtggg gtattaatgt ttaattacct
1321 ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat gtacccatac
1381 gatgttccag attacgcttc gccgaagaaa aagcgcaagg tcgaagcgtc cgacaagaag
1441 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
1501 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
1561 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
1621 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
1681 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
1741 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
1801 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
1861 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
1921 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
1981 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
2041 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
2101 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
2161 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
2221 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
2281 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
2341 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
2401 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
2461 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
2521 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
2581 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
2641 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
2701 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
2761 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
2821 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
2881 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
2941 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
3001 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
3061 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
3121 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
3181 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
3241 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
3301 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
3361 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
3421 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
3481 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
3541 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
3601 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
3661 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
3721 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
3781 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
3841 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
3901 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
3961 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
4021 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
4081 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
4141 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
4201 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
4261 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
4321 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
4381 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
4441 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
4501 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
4561 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
4621 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
4681 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
4741 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
4801 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg
4861 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
4921 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
4981 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
5041 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac
5101 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
5161 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
5221 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
5281 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
5341 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
5401 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
5461 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
5521 ctgggaggcg acagccccaa gaagaagaga aaggtggagg ccagctaaga attctaacta
5581 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
5641 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
5701 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
5761 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag cggccgcagg
5821 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
5881 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
5941 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
6001 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
6061 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
6121 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
6181 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
6241 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
6301 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
6361 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
6421 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
6481 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
6541 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
6601 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
6661 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
6721 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
6781 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
6841 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
6901 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
6961 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
7021 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
7081 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
7141 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
7201 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
7261 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
7321 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
7381 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
7441 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
7501 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
7561 tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc
7621 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
7681 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
7741 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
7801 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
7861 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
7921 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
7981 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
8041 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
8101 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
8161 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
8221 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
8281 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
8341 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
8401 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
8461 gtgatgctcg tcaggggggc ggagcctatg gaaa
//
